All-optical electrophysiology 

 

J. H. Hou JH, J. M. Kralj, A. D. Douglass, F. Engert, A. E. Cohen, "Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents", Front. Physiol., 2014 | doi: 10.3389/fphys.2014.00344

 

D. Hochbaum*, Y. Zhao*, S. Farhi, N. Klapoetke, C. A. Werley, V. Kapoor, P. Zou, J. M. Kralj, D. Maclaurin, N. Smedemark-Margulies, J. Saulnier, G. L. Boulting, C. Straub, Y.K. Cho, M. Melkonian, G. K-S. Wong, D. J. Harrison, V. Murthy, B. Sabatini, E. S. Boyden, R. E. Campbell, A. E. Cohen, “All optical electrophysiology in mammalian neurons using engineered microbial rhodopsins”, Nature Methods, 2014, doi:10.1038/nmeth.3000

 

P. Zou, Y. Zhao, A. D. Douglass, D. R. Hochbaum, D. Brinks, C. A. Werley, D. J. Harrison, R. E. Campbell, A. E. Cohen, “Bright and fast voltage reporters across the visible spectrum via electrochromic FRET (eFRET)”, arXiv.org (2014) http://arxiv.org/abs/1403.4636

V. Venkatachalam, D. Brinks, D. Maclaurin, D. Hochbaum, A. E. Cohen, “Flash Memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin,” J. Am. Chem. Soc. (2014) Vol. 136, pp. 2529-2537
 

J. Hou, V. Venkatachalam, A. E. Cohen, “Temporal dynamics of microbial rhodopsin fluorescence reports absolute membrane voltage,” Biophysical Journal, (2014) Vol. 106, pp. 639-648

J. Park*, C. A. Werley*, V. Venkatachalam, S. Dib-Hajj, S. Waxman, A. E. Cohen, “Screening fluorescent voltage indicators with spontaneously spiking HEK cells,” PLoS One, (2013) Vol. 8, pp. e85221

D. Maclaurin*, V. Venkatachalam*, H. Lee, A. E. Cohen (*Co-first authors), “Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin,” PNAS, (2013) Vol. 110, pp. 5939-5944

J. Kralj*, A. D. Douglass*, D. R. Hochbaum*, D. Maclaurin, A. E. Cohen (*Co-first authors), “Optical recording of action potentials in mammalian neurons using a microbial rhodopsin,” Nature Methods, (2012) Vol. 9, pp. 90-95

J. Kralj, D. R. Hochbaum, A. D. Douglass, A. E. Cohen, “Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein,” Science, (2011) Vol. 333, pp. 345-348

 

 

Stem cell biology

 

J. S. Toma, B. C. Shettar, P. H. Chipman, D. M. Pinto, J. P. Borowska, J. K. Ichida, J. P. Fawcett, Y. Zhang, K. Eggan, V. F. Rafuse, "Motoneurons derived from induced pluripotent stem cells develop mature phenotypes typical of endogenous spinal motoneurons", J Neurosci. 2015 Jan 21;35(3):1291-306. doi: 10.1523/JNEUROSCI.2126-14.2015.

 

B. J. Wainger, E. D. Buttermore, J. T. Oliveira, C. Mellin, S. Lee, W. A. Saber, A. J. Wang, J. K. Ichida, I. M. Chiu, L. Barrett, E. A. Huebner, C. Bilgin, N. Tsujimoto, C. Brenneis, K. Kapur, L. L. Rubin, K. Eggan, C. J. Woolf, "Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts", Nat Neurosci. 2015 Jan;18(1):17-24. doi: 10.1038/nn.3886. Epub 2014 Nov 24.

 

A. S. de Boer, K. Koszka, E. Kiskinis, N. Suzuki, B. N. Davis-Dusenbery and K. Eggan, "Genetic validation of a therapeutic target in a mouse model of ALS," 6 August 2014, Vol. 6 no. 248 pp. 248ra104. DOI:10.1126/scitranslmed.3009351 248ra104

 

j. K. Ichida, J. TCW, L. A. Williams, A. C. Carter, Y. Shi, M. T. Moura, M. Ziller, S. Singh, G. Amabile, C. Bock, A. Umezawa, L. L. Rubin, J. E. Bradner, H. Akutsu, A. Meissner and K. Eggan, "Notch inhibition allows oncogene-independent generation of iPS cells," Nat Chem Biol. 2014 Aug;10(8):632-9. doi: 10.1038/nchembio.1552. Epub 2014 Jun 22.

 

E. Kiskinis, J. Sandoe, L. A. Williams, G. L. Boulting, R. Moccia, B. J. Wainger, S. Han, T. Peng, S. Thams, S. Mikkilineni, C. Mellin, F. T. Merkle, B. N. Davis-Dusenbery, M. Ziller, D. Oakley, J. Ichida, S. Di Costanzo, N. Atwater, M. L. Maeder, M. J. Goodwin, J. Nemesh, R. E. Handsaker, D. Paull, S. Noggle, S. A. McCarroll, J. K. Joung, C. J. Woolf, R. H. Brown and K. Eggan, “Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1,” Cell Stem Cell, (2014) Vol. 14: Issue 6, pp. 781-795

 

B. J. Wainger, E. Kiskinis, C. Mellin, O. Wiskow, S. S. Han, J. Sandoe, N. P. Perez, L. A. Williams, S. Lee, G. Boulting, J. D. Berry, R. H. Jr Brown, M. E. Cudkowicz, B. P. Bean, K. Eggan and C. J. Woolf, “Intrinsic membrane hyperexcitability of amyotrophic lateral sclerosis patient-derived motor neurons,” Cell Reports, (2014) Vol. 7: Issue 1, pp. 1-11

 

C. S. Navara, J. Hornecker, D. Grow, S. Chaudhari, P. J. Hornsby, J. K. Ichida, K. Eggan and J. R. McCarrey, “Derivation of induced pluripotent stem cells from the baboon: a nonhuman primate model for preclinical testing of stem cell therapies,” Cellular Reprogramming, (2013) Vol. 15: Issue 6, pp. 495-502

 

E. Y. Son, J. K. Ichida, B. J. Wainger, J. S. Toma, V. F. Rafuse, C. J. Woolf and K. Eggan, “Conversion of mouse and human fibroblasts into functional spinal motor neurons,” Cell Stem Cell, (2011) Vol. 9: Issue 3, pp. 205-18

 

C. Bock, E. Kiskinis, G. Verstappen, H. Gu, G. Boulting, Z. D. Smith, M. Ziller, G. F. Croft, M. W. Amoroso, D. H. Oakley, A. Gnirke, K. Eggan and A. Meissner, “Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines,” Cell, (2011) Vol. 144: Issue 3, pp. 439-52

 

G. L. Boulting, E. Kiskinis, G. F. Croft, M. W. Amoroso, D. H. Oakley, B. J. Wainger, D. J. Williams, D. J. Kahler, M. Yamaki, L. Davidow, C. T. Rodolfa, J. T. Dimos, S. Mikkilineni, A. B. MacDermott, C. J. Woolf, C. E. Henderson, H. Wichterle and K. Eggan, “A functionally characterized test set of human induced pluripotent stem cells,” Nature Biotechnology, (2011) Vol. 3, pp. 279-86

 

F. P. Di Giorgio, G. L. Boulting, S. Bobrowicz and K. Eggan, “Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation,” Cell Stem Cell, (2008) Vol. 3: Issue 6, pp. 637-48

 

J. T. Dimos, K. T. Rodolfa, K. K. Niakan, L. M. Weisenthal, H. Mitsumoto, W. Chung, G. F. Croft, G. Saphier, R. Leibel, R. Goland, H. Wichterle, C. E. Henderson and K. Eggan, “Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons,” Science, (2008), Vol. 321: Issue 5893, pp. 1218-21